The Laboratory Robotics Interest Group Discussion Mail List is for comments or questions about Laboratory Robotics and Automation. You receive messages...
lrig-discussion@yahoo...
Jan 1, 2003 3:23 pm
2211
Hi there, Our experience here with cell factories has been a good one. With spinners there is the issues of minimizing the shear and aerating too much or too ...
Hello Everyone, I am trying to scale up our efforts to freeze down cell stocks and am looking for suppliers of equipment that can dispense cell suspensions...
I am working on a glow-luminescence application and observing an edge effect in 96-well plates—-high values on the edges, higher values in the corners....
Eric, Interesting that you should bring up this topic. We are experiencing the same issues with our glow luminescence assays in 384-well format. I'm very...
Eric, Douglas, After conferring with some of my colleagues here at Promega, I learned that we had a similar problem with expressing luciferase in E. coli...
Eric, We have coined your observed effects "THE RING OF FIRE" The solution would be to look into validating your assays using different plate sources. I can...
Hi Eric, Jared's reply is along the lines of a mutual solution to the problem. If the issue if temperature, one solution for the problem is to use a 96-well...
Even though evaporation may not be the direct cause of this effect, placing the plate in high humidity immediately after dispensing and during incubation, no...
Randy Engler
Randy.Engler@...
Jan 9, 2003 12:34 am
2219
Hello Group! I am looking for a 96 pin tool for a Tecan TEMO... can anyone recommend a supplier for this other than Tecan. I look forward to your comments. ...
Rich, Check out V&P Scientific. They designed some pin tools for our Quadra that would probably fit on the Tecan as well. Troy Smith Tomtec (925) 457-6448 ...
Troy Smith
troysmith619@...
Jan 9, 2003 3:51 am
2221
Eric, I have some experience with BMG readers, and my guess would be that optics, rather than temperature, is the culprit. While the LUMIstar is not in the...
G. S. Hudson
gshudson@...
Jan 9, 2003 4:14 am
2222
Hi Paul, Together with Computype (www.computype.com), we are working on a vial processing machine that seems to fit your requirements. The machine is designed...
Mike Theran
mtheran@...
Jan 9, 2003 8:01 pm
2223
I think Gordons explanation of reflection from the edges of the plate sounds most reasonable. So I wonder if you could see this edge-effect also with black...
Griesmayr, Gottfried
gottfried.griesmayr@...
Jan 9, 2003 8:01 pm
2224
One more note: Could it be the oxygen some times? When using a lid and with cellular samples it could be an issue. Gottfried ...
Griesmayr, Gottfried
gottfried.griesmayr@...
Jan 9, 2003 8:02 pm
2225
Rich, A well known vendor will be releasing a pin tool for the Tecan TeMO at LabAutomation next month. Marc Feiglin CTO- Drug Discovery Tecan ... From:...
It is possible to incubate at increased oxygen tension in cellular assays. If you specify that you need O2 control in an incubation system, you can get it. ...
Randy Engler
Randy.Engler@...
Jan 11, 2003 7:44 pm
2227
Dear Group, has anyone yet experienced a dependency of the pipetted volumes from humidity ? We see large volume deviations at the current dry winter air. We...
u.taibon@...
Jan 11, 2003 7:44 pm
2228
I have not seen any pipetting variations due directly to relative humidity but I have observed build up of static charge on pipette tips (and boxes) in dry...
Steve Fillers
steve.fillers@...
Jan 13, 2003 5:12 pm
2229
Apologies for being slightly off-topic: We've had very good results eliminating static charge in some sensitive areas by installing ion generators. This has...
petek@...
Jan 13, 2003 6:40 pm
2230
Hi, I'm not really happy with the software which is controlling my microplate reader and i would like to develop an interface to control it (with Visual Basic...
Thomas, Each manufacturer may use a different command set to control their readers, and should be willing and able to supply it to you, or at least that's...
Mark Russo is the champion in this field. He has written the most authoritative book on Visual Basic in the laboratory. Mark and Martin Echols are offering a...
Thanks for this info. Do you think it's possible to "catch" the control protocol using a COM port analyzer or something like that ? Best regards, Thomas...
You will have to get the protocol from the device manufacturer or you can use a protocol analyzer to obtain the protocol. You can see more at our website: ...
kebaldwin@...
Jan 14, 2003 12:19 am
2235
Hello, You can use hardware and software to sniff the commands from the port, just type something like 'com port sniffer' into google. However beware that...
Hi, In our group we are doing HTS using Packard workstations with 384 heads equipped with plastic tips. This equipement is also used for wet pipetting of low...
You really should get the protocol from the instrument manufacturer. It could be rather hard to reverse engineer the protocol using a COM port analyzer....
Hi Johannes. In our experience uncoated stainless steel is not suitable for handling compounds in DMSO. Old Hydra's had raw stainless steel tips which were...
Thomas, What is the brand and model reader you are using? Many system integrators (Beckman, Hudson, Tecan, Thermo-CRS, Zymark...others) have written their own...
