...For those interested... We have discovered several things since I sent this note: * The Beckman AP96 20ul nonsterile tips only survive about 20-30 washes...
Jung, Mark T.
MARK.T.JUNG@...
Nov 2, 2003 8:04 pm
3132
Hi Group, How do I validate the pipetting accuracy/precision of a 10-fold dilution in 96-well plates using a PBS-Casein-Tween20 dilution buffer? I would like...
Thanks Steve. I was planning to first do the fluorometery because I can evaluate the results instantly then refine my washing protocols. Later, I will run...
Hi Morten, I have never ever worked with PBS-Casein-Tween20 dilution buffer. Is there no possibility to get rid of your bubbles using a...
Matthias Schick
schick@...
Nov 3, 2003 7:39 pm
3135
Cover and spin your plates (100xg for a minute will be probably sufficient) after you made the dilution and before reading the plate. That should take care of...
oh, by the way, another thought that just came to me was to use a very bright label that you can do extra dilution into a non - formy diluent... assuming that...
Hi Morten, RNAture, Inc. makes a tool for liquid handlers that helps prevent bubble formation during dispense steps (among other things). Please click here...
Elliott Gorfain
egorfain@...
Nov 3, 2003 8:15 pm
3138
Dear List i would like to know if you would have a preference between TopCount or MicroBetaJet for 3H-based radioligand filtration binding on membrane in 96...
Hello, You should get a pretty honest answer from the sales(wo)man as the same company sells both pieces of kit. The many faces of Perkin Elmer!! Cheers, Neil...
Hello LRIGers, I am looking for a microtiter plate shaker with accurate speed control. I prefer an orbital mixer that has a fairly rapid speed of mixing. I...
John Thomas Bradshaw
JBradshaw@...
Nov 5, 2003 2:13 pm
3141
Hi Morten, Well, spinning is one possibility (as some alreay responded), but with Tween this might be a problem, as these small bubbles often are resistant to...
Hi Emmanuell, No alternative as far as I know, both instruments are from PE. Sales recommendation will probably depend on origin of the sales guy - former...
I would discuss this with GlasCol out of Terre Haute, IN. They do great work and will work with you on your needs. Their shakers also can integrate with...
Try Big Bear Automation. They make a very nice plate shaker with RS232 communications port. You can control on/off, acceleration, velocity, etc. It also...
Hi, Teleshake from H+P Labortechnik is worth looking on. http://www.hp-lab.de/englisch/shaker/shaker_01.html technical information: ...
Matthias Schick
schick@...
Nov 5, 2003 4:33 pm
3146
Hi all, I was wondering if there was someone here who could help me. A few months ago we had a TECAN PW384 (with WinWash v2.11) installed in with our TECAN...
Hi All, We are looking for a microplate hotel that will shake the microplates in an orbital rotation (not linear) and can be integrated to an articulated arm....
Hi John, You got already the notice for H+P (www.hp-lab.de). They do not have a dig-display, but a real fast shaker (up to about 8kHz, robotfriendly, lauched...
Brian, Jouan Robotics has a 44 and 200 plate capacity shaking hotel. You can contact me off-line for more information. Paul Murphy Jouan Robotics 617-413-9001 ...
Brian, Liconic offers a 44 position hotel with an orbital shaker. It is called the LPX44. The shake is adjustable upto 600RPM. I believe this is the same...
Brian, Yes, we work with Liconic to develop this product. It has a 1.5 amplitude and is adjustable to 600 rpm. We have easily integrated it with CRS and Hudson...
Hello, There is a company called DPC in North Wales, they use an electro magnetic shaker rather than an offset motor. Therfrefore it can be controlled in...
Beckman Coulter also uses the DPC MicroMix 5 Shaker on the Biomek 2000. It's a robust unit and is automation friendly. Bob Tepe rjtepe@......
rjtepe@...
Nov 7, 2003 8:31 pm
3154
John, At Zymark, we integrate the H+P Variomag shaker and find it to provide excellent performance and control..., both linear and orbital shaking from...
Hello, Has anyone on this group tried doing some work with .NET and Excel (2002)? If you have, could you gimmie a shout please - I've bashing my head against...
Dear John, Not to promote, but to inform. Please take a look at the high speed TEOS shakers from (www.techelan.com) The speed on the remote controlled unit...
Hello all LRIGers Does anybody know about a liquid handler that can do columnwise serial dilutions in a 384 well plate? I need something that can give me CVs...
Hi Max - We consider this capability to be 'table stakes' for current liquid handlers. Please take a look at the Sciclone ALH 3000. We have published ...
Ed Alderman
ed.alderman@...
Nov 12, 2003 3:32 am
3159
I am the product manager from Molecular Devices for the Analyst multi-mode reader. Not trying to sell you anything but we do have an instrument called...
I think you need to clarify your method and you expectations a little more before anyone can give you a proper answer about their equipments capability. 1.)...
