Hello Anne, We have tested dozens of readers from all over the world, and have found that the most expensive instruments are rarely the "best." My ...
G. S. Hudson
gshudson@...
Dec 1, 2003 2:36 pm
3204
Group, We have developed a system that allows us to wash tips with very good results (i.e. no carryover). We work with a lot of protein and find that the tips...
Gregg, We use Sigmacote (organopolysiloxane) quite often when working with peptides that are prone to non-specific binding to labware surfaces. We have seen a...
The Laboratory Robotics Interest Group Discussion Mail List is for comments or questions about Laboratory Robotics and Automation. You receive messages...
lrig-discussion@yahoo...
Dec 1, 2003 9:20 pm
3207
Hi Everyone, I am interested in the Tecan Power Washer 384 for cell-based assays to be used either as a stand alone instrument in a BioSafety Hood or on the...
Hi Everyone, I am interested in comments related to plate readers. We are looking for something that is very versatile, since it will be part of a shared core...
Paul, BMG has a new HTS reader, The PHERAstar. I'll keep the advertisement short: It has four PMTs--two used for fluorescence intensity, two optimized for...
Paul, Obviously depending in the reader some technologies might not be possible so you're goingto have to either make a compromise or go for a reader which ...
... Paul, we use both washers and I can recommend the Elx-405. It's way cheaper than the PW384 and we experienced several errors with the PW384 (especially...
Paul, For cell based assays I would recommend the Biotek washer. The main advantage I found are the angled dispensing pins which direct the liquid to the side...
Hi Paul, I spent most of a day evaluating the Berthold fluorometer at a trade show several months ago. We read several different models of our Fluorescence...
G. S. Hudson
gshudson@...
Dec 2, 2003 5:21 pm
3214
Can someone help me troubleshoot? I have an Agilent 1100 HPLC equipped with an ELSD and DAD. I am experiencing intermittent negative peaks in my UV traces....
Hello B M; If you think the culprit is air in the detector (out gassing), disconnect the outlet line from the UV detector if its attached to the ELSD and...
Hello B M; One trouble shooting suggestion if you haven't done this yet is to stop the flow within the system and monitor the baseline on the UV. If you still...
The most common reason for negative peaks is that the UV absorbance of your sample is less than that of your mobile phase. Have you examined the spectra of...
I think the new Ultra's have both top and bottom reading. -Mike Fabrice Turlais <fabrice.turlais@cambridgeant To: lrig-discussion@yahoogroups.com ...
Michael_Shanler@...
Dec 2, 2003 9:04 pm
3219
Thanks Mike for the clarification. Bottom read fluorescence IS available on the new Ultra Evolution. Sincerely, Mike ... From: Michael_Shanler@......
Mike.Cianci@...
Dec 3, 2003 2:35 am
3220
Look at Molecular Devices, the Gemini EM is a very good reader, with a very to use software, that gives opportunity for excel data handling or to set up your...
I need a polyethylene 96 well UV transparent microtiter plate (for use in a spectrophotometer). Anyone know if they exist and who makes them? Thanks [Non-text...
Dear Chris, don't know whether these are made from PE, but there are at least two vendors: - Greiner Bio-one UVStar ##655801, 781801 - Corning UV Microplates...
I am not aware of any PP plates that are UV transparent. Nunc, Greiner and Corning may offer plates made of other polymers that are UV transparent. We stock a...
Fred Spike
fred@...
Dec 5, 2003 4:37 pm
3224
Chris, We don't make a polyethylene 96 well plate but we do make a quartz bottom plate. Cost is in the $25/plate range. Cheers, Kevin ... From: Chris Reichard...
Hi everyone, I am Prabhakaran. I am not a serious researcher like you folks. I am medicine resident doing part time research. Hence I am running into truouble...
Dear Dr. Prabhakaran, While at the last LRIG vendor exhibition, I was very impressed by the Covaris E-Series sample preparation units. They work on a...
Dear Chris, Some years ago we developed quite acceptable UV transmitting (>340nm) 96MTPs with Polyfiltronics (now Whatman) for Fura-2 stained cellular calcium...
Hello, Prabakarang, I cannot claim to have experience in doing specifically what you ask about, but I have done a lot of grinding. As I see the problem, you...
Tom McCloud
Tom@...
Dec 8, 2003 6:43 pm
3230
Hi Everyone, I am interested in any comments on two thermal plate sealers: Velocity11's PlateLoc Thermal Plate Sealer Versus ABgene's ALPS 300™ automated...
Hi Prabhakaran: I think you have several options, depending on your throughput. If you are doing a limited number of samples, then a micro tissue grinder...
David Burden
david.burden@...
Dec 8, 2003 8:46 pm
3232
Hi all I am currently trying to find some filters that can be attached to the supply tubing for the ELX50 automated plate washer. Does anyone have any ideas? ...
